. Kramer and A . M . Corbett skeletal muscle triads and terminal cisternae
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Morphology of isolated triads
The triad is the junctional association of transverse tubule with sarcoplasmic reticulum terminal cisternae. A procedure for the isolation of highly enriched triads from skeletal muscle has been described in the previous paper. In the present study, the structural features of isolated triads have been examined by thin-section, negative-staining, and freeze-fracture electron microscopy. In isola...
متن کاملIdentification of a constituent of the junctional feet linking terminal cisternae to transverse tubules in skeletal muscle
This study describes the biochemical composition of junctional feet in skeletal muscle utilizing a fraction of isolated triad junctions. [3H]Ouabain entrapment was employed as a specific marker for T-tubules. The integrity of the triad junction was assayed by the isopycnic density of [3H]ouabain activity (24-30% sucrose for free T-tubules, 38-42% sucrose for intact triads). Trypsin, chymotrypsi...
متن کاملRole of triadin in the organization of reticulum membrane at the muscle triad.
The terminal cisternae represent one of the functional domains of the skeletal muscle sarcoplasmic reticulum (SR). They are closely apposed to plasma membrane invaginations, the T-tubules, with which they form structures called triads. In triads, the physical interaction between the T-tubule-anchored voltage-sensing channel DHPR and the SR calcium channel RyR1 is essential because it allows the...
متن کاملInteraction of glyceraldehyde-3-phosphate dehydrogenase with isolated microsomal subfractions of skeletal muscle.
A protein of subunit Mr 34,000 (corrected here and subsequently to 36,000) has been isolated from a muscle homogenate which catalyzes the formation of the triad junction from isolated transverse tubules and terminal cisternae. This protein is identified as glyceraldehyde-3-phosphate dehydrogenase on the basis of N-terminal amino acid sequence, amino acid composition, and enzymic activity. The o...
متن کاملPreparation and morphology of sarcoplasmic reticulum terminal cisternae from rabbit skeletal muscle
We have developed a procedure to isolate, from skeletal muscle, enriched terminal cisternae of sarcoplasmic reticulum (SR), which retain morphologically intact junctional "feet" structures similar to those observed in situ. The fraction is largely devoid of transverse tubule, plasma membrane, mitochondria, triads (transverse tubules junctionally associated with terminal cisternae), and longitud...
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